Vascular smooth muscle cell differentiation – 2010

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Vascular smooth muscle cell differentiation-2010

Vascular smooth muscle cells have attracted considerable interest as a model for a flexible program of gene expression. This cell type arises throughout the embryo body plan via poorly understood signaling cascades that direct the expression of transcription factors and microRNAs which, in turn, orchestrate the activation of contractile genes collectively defining this cell lineage. The discove...

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Experiments were performed to determine the role of reactive oxygen species (ROS) in regulating vascular smooth muscle cell (VSMC) phenotype. After quiescence, cultured human VSMCs increased their expression of differentiation proteins (alpha-actin, calponin, and SM1 and SM2 myosin), but not beta-actin. ROS activity, determined using the H(2)O(2)-sensitive probe dichlorodihydrofluorescein (DCF)...

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Sympathetic innervation promotes vascular smooth muscle differentiation.

The sympathetic nervous system (SNS) is an important modulator of vascular smooth muscle (VSM) growth and function. Several lines of evidence suggest that the SNS also promotes VSM differentiation. The present study tests this hypothesis. Expression of smooth muscle myosin (SM2) and alpha-actin were assessed by Western analysis as indexes of VSM differentiation. SM2 expression (normalized to al...

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The Role of MicroRNAs in Vascular Diseases; Smooth Muscle Cell Differentiation and De-Differentiation

Vascular smooth muscle cells (VSMCs) undergo two important physiological processes such as differentiation (contractile state) and dedifferentiation (proliferative state) during vascular diseases. In general, a prerequisite for pathological and physiological vascular remodeling processes such as atherosclerosis, hypertension, and restenosis after angioplasty is the phenotype alteration of VSMCs...

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The angiotensin II type 2 (AT2) receptor is transiently expressed at late gestation in the fetal vasculature, but its expression rapidly declines after birth. We have previously demonstrated that the expression of this receptor mediates decline in vascular DNA synthesis that occurs at this stage of vascular development. To examine further the role of the AT2 receptor in vasculogenesis, we have ...

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ژورنال

عنوان ژورنال: Journal of Biomedical Research

سال: 2010

ISSN: 1674-8301

DOI: 10.1016/s1674-8301(10)60026-7